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F3165
Sigma
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
clone M2, purified immunoglobulin, buffered aqueous solution
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | For detection of FLAG fusion proteins by immunoprecipitation, immunoblotting, or EIA. |
| Other Notes | To view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature. |
| Specificity | Binds to the FLAG® epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent. |
| Legal Information | ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| antibody form | purified immunoglobulin |
| clone | M2 (monoclonal) |
| form | buffered aqueous solution |
| application(s) | immunoblotting (chemiluminescent): 10 μg/mL |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Related product | F9291, Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse |
| P7582, Amino-terminal FLAG-BAP™ Fusion Protein | |
| CE0050, CelLytic™ MEM Protein Extraction Kit | |
| F1804, Monoclonal ANTI-FLAG® M2 antibody produced in mouse - F1804 is a peptide affinity-purified version of F3165. | |
| F8775, Formaldehyde solution |
References
| reference | Li, Feng-Qian, et al., Chibby cooperates with 14-3-3 to regulate β-catenin subcellular distribution and signaling activity J. Cell Biol. 7th ed., 181, 1141-1154, (2008) |
